Potassium Dichromate Reference

For assessing the absorbance scale of your UV spectrophotometer

Description Potassium dichromate in perchloric acid sealed in a quartz cell
Primary Usage Test the absorbance scale of UV spectrophotometers
Useable Range 235nm to 350nm ( 20mg/L to 100mg/L) or 430nm (600mg/L)
Physical Configuration Far UV quartz cell that has been permanently sealed by heat fusion
NIST Traceability Each set is supplied with a certificate of analysis and NIST Traceability. The 600mg/L is used for compliance with the European Pharmacopoeia and is also traceable to NIST. All certificates are supplied by our ISO 9001, ISO 17025 and UKAS accredited laboratory.

Set of 5 concentrations and a blank
Catalog Number: RM-0204060810

Product Description

Potassium Dichromate solvated in dilute (0.001N) perchlorate acid has been found to be the best method for testing the absorbance scale and linearity of spectrophotometers in the UV. Starna purchases the potassium dichromate crystals directly from NIST (SRM 935a). We then make up the solutions as per the instructions from NIST and permanently heat fuse seal the solutions in UV quartz spectrophotometer cells.

The filled cells are tested and certified against the expected values from NIST and a certificate is provided with each set of cells which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for the quality control that is established in your laboratory. Each set is supplied with a sealed blank cell that was used for the certified measurement.

Suggestions for Use

Measure the absorbance of the potassium dichromate cells at the four wavelengths specified by NIST. These wavelengths for the 20 to 100mg/L solutions are 235, 257, 313 and 350nm and the 600mg/L solution measurement is made at 430nm. The absorbance scale is verified by assuring that the absorbance readings on your instrument are within your instruments tolerance to the readings on the certificate supplied with the reference set. To evaluate linearity, plot a graph of concentration against absorbance for each of the four wavelengths. The 600mg/L solution is used for European Pharmacopoeia compliance with a single wavelength measurment.

Scan of Potassium Dichromate set

Linearity Graph

Approximate Absorbances

Concentration 235nm 257nm 313nm 350nm 430nm
20 mg/L 0.2430 0.2810 0.0950 0.2090 n/a
40 mg/L 0.4920 0.5720 0.1920 0.4260 n/a
60 mg/L 0.7410 0.8620 0.2890 0.6340 n/a
80 mg/L 0.9960 1.1590 0.3850 0.8530 n/a
100 mg/L 1.2430 1.4480 0.4800 1.0690 n/a
600mg/L n/a n/a n/a n/a 0.950

Interpertation of Results

Compare the absorbance values from your instrument with the expected values on the certificate supplied with the reference set. If the readings are within the expected range for your instrument then your absorbance scale is correct. If not, consult your service techincian and have the instrument evaluated

Quality program

Each time that the analysis is repeated you can compare the new results with the previous results to check for any variance. If your instrument needs service because of a change in resolution, the historical data will greatly assist the service technician.

How to Order

Catalog Number Description Price each
Linearity Dichromate set
RM-0204060810 Potassium Dichromate Reference set, 5 Concentrations US$ 1,800.00
Linearity Dichromate set with 430nm European Pharmacopoeia Compliance
RM-020406081060 Potassium Dichromate Reference set, 6 Concentrations US$ 2,180.00
Single concentrations:
RM-02 Potassium Dichromate Reference set, 20mg/L US$ 750.00
RM-04 Potassium Dichromate Reference set, 40mg/L US$ 750.00
RM-06 Potassium Dichromate Reference set, 60mg/L US$ 750.00
RM-08 Potassium Dichromate Reference set, 80mg/L US$ 750.00
RM-10 Potassium Dichromate Reference set, 100mg/L US$ 750.00
Single concentration, European Pharmacopoeia Compliance:
RM-60 Potassium Dichromate Reference set, 600mg/L US$ 750.00

Starna Cells, Inc PO Box 1919 Atascadero, CA 93423 USA
Phone: 805-466-8855 Fax: 805-461-1575 e-mail: sales@starnacells.com