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Holmium Reference for Wavelength in the Visible and UV
For assessing the wavelength accuracy of your spectrophotometer
Description Holmium oxide (4%) in perchloric acid (10%) sealed in a quartz cell
Primary Usage Verify the wavelength scale of UV/visible spectrophotometers
Useable Range 240nm to 650nm, 14 traceable absorbance peaks
Physical Configuration Far UV quartz cell that has been permanently sealed by heat fusion
NIST Traceability Each set is supplied with a Certificate of Analysis and NIST Traceability
ISO Accredited Accredited by UKAS ISO 17025, ISO Guide34 and ISO 9001

Product Description

Holmium has been used as a wavelength reference for many years and now we have extended the use by creating a filled and sealed quartz cell which allows you to verify your wavelength scale into the Ultraviolet. Holmium offers sharp, stable peaks over the range of 219nm to 650nm. Holmium perchlorate is used to assure that the wavelength scale of your instrument is within the manufacturers tolerances to the actual wavelength being measured. The use of the filled quartz cell assures that the optical configuration of your quality control is exactly the same as for a normal analysis.

UV RegionVisible RegionVisible Region
241.0333.5451.1
249.9345.6467.8
278.2361.4485.2
287.1385.4536.5
416.1640.6

Suggestions for Use

Holmium presents a wide range of crisply resolvable peaks which are easily used to correlate the wavelength indicator on your spectrophotometer to the known Holmium peaks. The first procedure should be to scan the cell over the useable range to assure that all of the peaks can be resolved. Alter the slit width and scan rate to produce a useable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances for the wavelength of your spectrophotometer. If not, get a service technician to check and, if necessary, adjust your instrument. Holmium is measured against an air blank.

Periodically check the spectra, at a minimum in the area where you are doing your analysis. The periodic use of your Holmium cell will build a log of your instrument's wavelength accuracy for use with certification and troubleshooting should the correlation change over time.


Absorbance Scan of Holmium


Transmission Scan of Holmium


Interpertation of Results

Compare the wavelength peaks from your instrument with the expected values on the certificate supplied with the reference set. If the readings are within the expected range for your instrument then your wavelength scale is correct. If not, consult your service techincian and have the instrument evaluated

Quality program

Each time that the analysis is repeated you can compare the new results with the previous results to check for any variance. If your instrument needs service because of a change in resolution, the historical data will greatly assist the service technician.

How to Order

Catalog Number Description Price each
RM-HL Wavelength Reference Cell, Holmium Perchlorate,
NIST traceable and ISO 17025
US$ 550.00
Download MSDS Sheet for RM-HL